南方医科大学学报Issue(7)：965-968,4.DOI:10.3969/j.issn.1673-4254.2014.07.10
ppk1基因缺失对脑膜炎大肠杆菌K1株生物学功能的影响
Changes of biological behavioral of E. coli K1 after ppk1 gene deletion
摘要
Abstract
Objective To study the changes in biological behaviors of meningitis E. coli K1 strain E44 after deletion of polyphosphate kinase 1 (ppk1) gene and explore the role of ppk1 in the pathogenesis of E. coli K1-induced meningitis. Methods The wild-type strain E. coli K1 and ppk1 deletion mutant were exposed to heat at 56℃for 6 min, and their survival rates were determined. The adhesion and invasion of the bacteria to human brain microvascular endothelial cells (HBMECs) were observed using electron microscopy and quantitative tests. HBMECs were co-incubated with wild-type strain or ppk1 deletion mutant, and the cytoskeleton rearrangement was observed under laser scanning confocal microscope. Results The survival rate of the ppk1 deletion mutant was significantly lower than that of the wild-type strain after heat exposure. The ppk1 deletion mutant also showed lowered cell adhesion and invasion abilities and weakened ability to induce cytoskeleton rearrangement in HBMECs. Conclusion ppk1 gene is important for E.coli K1 for heat resistance, cell adhesion and invasion, and for inducing cytoskeletal rearrangement in HBMECs.关键词
脑膜炎/聚磷酸盐激酶1/大肠杆菌K1株/基因敲除Key words
meningitis/polyphosphate kinase/Escherichia coli/gene deletion引用本文复制引用
彭亮,潘嘉韵,罗苏,杨正慧,黄慕芳,曹虹..ppk1基因缺失对脑膜炎大肠杆菌K1株生物学功能的影响[J].南方医科大学学报,2014,(7):965-968,4.基金项目
国家自然科学基金青年项目（81200505）；国家级大学生创新创业训练计划项目（201212121092）；广东省大学生创新实验计划项目（1212111027） National Natural Science Foundation of China (81200505) （81200505）
