南方医科大学学报Issue(7)：1071-1074,4.DOI:10.3969/j.issn.1673-4254.2014.07.33
siRNA抑制CDK6表达影响鼻咽癌细胞增殖和细胞周期进展
siRNA-mediated CDK6 knockdown suppresses nasopharyngeal carcinoma cell growth and cell cycle transition in vitro
摘要
Abstract
Objective To assess the effect of small interfering RNA (siRNA)-mediated suppression of CDK6 expression on the proliferation and cell cycles of nasopharyngeal carcinoma (NPC) cells in vitro. Methods QRT-PCR was used to examine the differential expression of CDK6 in 30 NPC tissues and 18 normal nasopharyngeal tissues. A siRNA targeting CDK6 was transfected in NPC CNE2 cells, and MTT assay and flow cytometry were used to analyze the changes in cell proliferation and cell cycle distribution. Western blotting was used to examine the expressions of the cell cycle-related factors. Results Compared with normal nasopharyngeal tissues, NPC tissues showed an increased expression of CDK6 mRNA. Knocking down CDK6 expression obviously inhibited tumor cell growth and cell cycle transition from G1 to S phase and caused reduced expressions of CDK4, CCND1, and E2F1 and enhanced expression of the tumor suppressor p21. Conclusion NPC tissues overexpress CDK6. Knocking down CDK6 expression inhibits the growth and cell cycle transition of NPC cells in vitro by inhibiting the expressions of CDK4, CCND1, and E2F1 and upregulating tumor suppressor p21 expression.关键词
鼻咽癌/CDK6/siRNA/细胞增殖/细胞周期Key words
nasopharyngeal carcinoma/CDK6/small interfering RNA/cell proliferation/cell cycle引用本文复制引用
罗小鹏,夏琼,覃继新,黄永秩,刘津,王莹,王怀飞,陈家军..siRNA抑制CDK6表达影响鼻咽癌细胞增殖和细胞周期进展[J].南方医科大学学报,2014,(7):1071-1074,4.基金项目
广西自然科学基金 （）
