南方医科大学学报Issue(11)：1606-1610,5.DOI:10.3969/j.issn.1673-4254.2014.11.10
Toll样受体7在人胃癌细胞株中的表达及其激动剂诱导SGC-7901细胞发生凋亡的实验研究
Expression of Toll-like receptor 7 in gastric cancer cell lines and effects of TLR7 agonist on proliferation and apoptosis of SGC-7901 cells in vitro
摘要
Abstract
Objective To investigate the expression of Toll-like receptor 7 (TLR7) in gastric cancer cell lines and the effect of imiquimod, a TLR7 agonist, on the proliferation and apoptosis of SGC-7901 cells. Methods The protein expression levels of TLR7 were detected with Western blotting in 3 human gastric cancer cell lines (SGC-7901, HGC-27 and MKN-28). The cell line expressing the highest TLR7 level was exposed to different doses of imiquimod for 12-72 h and the cell viability was assessed with MTT assay. The cell apoptosis rate after 100μg/ml imiquimod treatment for 12 or 24 h was quantified by flow cytometry, and the ultrastructual changes of the cells were observed under electron microscope. The expression of apoptosis-related genes Bcl-2 and Bax were analyzed with real-time PCR. Results All the 3 cell lines expressed TLR7, among which SGC-7901 cells showed the highest expression level. TLR7 agonist imiquimod dose-and time-dependently reduced the viability of SGC-7901 cells. Exposure to 100μg/ml imiquimod for 24 h resulted in SGC-7901 cell apoptosis as shown by an increased ratio of early apoptotic cells and significant ultrastructural changes of the cells. Real-time PCR demonstrated that imiquimod treatment for 24 h caused a dose-dependent reduction of Bcl-2 mRNA expression and increment of Bax mRNA expression. Conclusion TLR7 protein is expressed in all the 3 gastric cancer lines and its agonist imiquimod can inhibit cell proliferation and induce apoptosis in SGC-7901 cells.关键词
胃癌/Toll样受体7/咪喹莫特/凋亡Key words
gastric cancer/Toll-like receptors 7/imiquimod/apoptosis引用本文复制引用
姜炅,董蕾,秦斌,郭晓燕,李红,史海涛,刘亚萍..Toll样受体7在人胃癌细胞株中的表达及其激动剂诱导SGC-7901细胞发生凋亡的实验研究[J].南方医科大学学报,2014,(11):1606-1610,5.基金项目
“十二五”国家科技支撑计划（2012BAJ18B03-03） National "Twelfth Five-Year" Plan for Science & Technology Support （）
