南方医科大学学报Issue(11)：1621-1626,1631,7.DOI:10.3969/j.issn.1673-4254.2014.11.13
大鼠骨髓间充质干细胞的分离与培养方法优化
Optimization of the method for isolating and culturing rat mesenchymal stem cells
摘要
Abstract
Objective To optimize the protocols for isolation and culture of mesenchymal stem cells from rat bone marrow (BMSCs). Methods BMSCs were isolated by adherence to plastic with frequent medium change and reduced trypsinization time. The cell growth curves were drawn and the surface markers of BMSCs were detected by flow cytometry. The cells were induced to differentiate into osteogenic, adipogenic, hepatic and cholic lineages. Results The cells isolated using this method were positive for CD29, CD44, and CD90 and negative for the hematopoietic surface markers CD45. The osteogenic and adipogenic differentiation of the BMSCs was verified by alkaline phosphatase staining, Alizarin red staining and Oil red staining. The cell subcultures up to passage 10 maintained capacities of differentiation into osteogenic and adipogenic lineages. The BMSCs induced with sequential addition of growth factors, cytokines and hormones differentiated into cells expressing hepatocyte-and cholangiocyte-specific markers. Conclusion The optimized method allows efficient isolation of homogenous populations of MSCs from rat bone marrow, which can be induced into multiple cell lineages.关键词
骨髓/间充质干细胞/分离与培养/肝细胞/胆管上皮细胞/大鼠Key words
bone marrow/mesenchymal stem cell/isolation and culture/hepatocytes/biliry epithelial cells引用本文复制引用
祝旭龙,颜谭,姚维杰,王永恒,程冲,向俊西,吕毅,高庆东,李建辉..大鼠骨髓间充质干细胞的分离与培养方法优化[J].南方医科大学学报,2014,(11):1621-1626,1631,7.基金项目
国家自然科学基金（81170455）；陕西省科技统筹创新工程项目（2011KJCL03-13） Supported by National Natural Science Foundation of China (81170455) （81170455）
