南方医科大学学报Issue(11)：1642-1645,4.DOI:10.3969/j.issn.1673-4254.2014.11.17
苯乙烯类菁染料pH荧光探针在细胞荧光成像中的应用及细胞毒性检测
Live cell fluorescent imaging and cytotoxicity assessment of pH fluorescent probe based on styrylcyanine dyes
摘要
Abstract
Objective To prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging. Methods The Probe 1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on the fluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as a pH fluorescence probe in living cell. Results Probe 1 emitted green fluorescence under neutral and basic conditions but orange fluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantly affecting the cell viability. Conclusion The pH-sensitive fluorescent probe prepared based on styrylcyanine possesses good ability of cell membrane permeation for live cell fluorescent imaging.关键词
苯乙烯类菁/pH荧光探针/细胞成像/细胞毒性Key words
styrylcyanine/pH fluorescent probe/cell imaging/cytotoxicity引用本文复制引用
黄丽霞,刘香,崔丹婷,许乙凯,游文玮,严轶琛,路新卫,刘瑞源..苯乙烯类菁染料pH荧光探针在细胞荧光成像中的应用及细胞毒性检测[J].南方医科大学学报,2014,(11):1642-1645,4.基金项目
国家自然科学基金（21244007，81271642）；教育部博士学科点专项科研基金（20114433110009） Supported by National Natural Science Foundation of China (21244007,81271642) （21244007,81271642）
