南方医科大学学报Issue(11)：1693-1696,1701,5.DOI:10.3969/j.issn.1673-4254.2014.11.29
曲格列酮上调PPAR-γ抑制宫颈癌HeLa细胞ICAM-1和MMP-9表达
Troglitazone suppresses ICAM-1 and MMP-9 expressions by up-regulating PPARγin HeLa cells
摘要
Abstract
Objective To investigate the effect of troglitazone, a proliferator-activated receptor-γ (PPARγ) agonist, on the expressions of intercellular adhesion molecule-1 (ICAM-1) and matrix metalloproteainse-9 (MMP-9) and cell proliferation in HeLa cells. Methods MTT assay was used to measure the cell viability at different time points (0, 24, 48 and 72 h) after exposure to troglitazone. RT-PCR and Western blotting were employed to detect the mRNA and protein expressions of ICAM-1, MMP-9 and PPARγin the cells. Electrophoretic mobility shift assay (EMSA) was performed to assess the changes in DNA binding activity of PPARγ. Results The viability of HeLa cells were time-dependently inhibited by troglitazone, which also significantly reduced the mRNA and protein expressions of ICAM-1 and MMP-9 and increased PPARγexpression. The effects of troglitazone in inhibiting the cell viability and reducing ICAM-1 and MMP-9 expressions were antagonized by the application of the PPARγantagonist GW9662. The result of EMSA also showed significantly increased DNA binding activity of PPARγin the cells exposed to troglitazone. Conclusions The PPARγagonist troglitazone can inhibit the expression of ICAM-1 and MMP-9 in HeLa cells by up-regulating PPARγ.关键词
HeLa细胞/曲格列酮/PPARγ/ICAM-1/MMP-9Key words
HeLa cells/troglitazone/proliferator-activated receptor-γ/intercellular adhesion molecule-1/matrix metalloprote-ainse-9引用本文复制引用
周晓霞,王智彪..曲格列酮上调PPAR-γ抑制宫颈癌HeLa细胞ICAM-1和MMP-9表达[J].南方医科大学学报,2014,(11):1693-1696,1701,5.基金项目
重庆市科技项目 （）
