南方医科大学学报Issue(12)：1733-1737,5.DOI:10.3969/j.issn.1673-4254.2014.12.05
外周血中血脑屏障损伤新型标记物脑微血管内皮细胞检测方法的建立
Establishment of a method for detecting peripheral blood circulating brain microvascular endothelial cells, a novel biomarker for blood-brain barrier injury
摘要
Abstract
Objective To establish a method for detecting circulating brain microvascular endothelial cells (cBMECs), a novel biomarker of blood-brain barrier (BBB) injury. Methods Blood samples were collected from 33 patients with AIDS encephalitis and 13 healthy subjects for detection of cBMECs, cECs and EPCs using magnetic affinity isolation and immune identification technology. Results The numbers of cBMECs, cECs and EPCs were significantly higher in the AIDS patients than in the control subjects (t=4.298, P<0.01; t=4.886, P<0.01; t=4.889, P<0.01). An significant association was also noted between HIV load and cBMEC number (r=0.928, P<0.01). Conclusion We have successfully established a method for detecting peripheral blood cBMECs, which can be of important value in non-invasive assessment of BBB injury.关键词
外周血/生物标记物/脑微血管内皮细胞/血脑屏障损伤Key words
peripheral blood/biomarker/circulating brain microvascular endothelial cells/blood-brain barrier injury引用本文复制引用
李妍,杜蕾,袁霖,陈德喜,邱嘉文,何肖龙,曹虹,黄胜和..外周血中血脑屏障损伤新型标记物脑微血管内皮细胞检测方法的建立[J].南方医科大学学报,2014,(12):1733-1737,5.基金项目
国家自然科学基金（81171644，81370740） Supported by National Natural Science Foundation of China(81171644,81370740) （81171644,81370740）
