南方医科大学学报Issue(12)：1738-1742,5.DOI:10.3969/j.issn.1673-4254.2014.12.06
基于3'RACE的胃癌细胞肿瘤相关基因的APA位点分析
Alternative polyadenylation site analysis of tumor-related genes based on 3'RACE in gastric cancer cells
摘要
Abstract
Objective To analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells. Methods We used 3'RACE to capture the APA sites of two tumor-related genes (HSP90αand SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database. Results We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs. Conclusions There are new mRNA isoforms of HSP90αand SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.关键词
胃癌/3'RACE/APAKey words
gastric cancer/3'RACE/alternative polyadenylation引用本文复制引用
赖登攀,陈健,康亚妮..基于3'RACE的胃癌细胞肿瘤相关基因的APA位点分析[J].南方医科大学学报,2014,(12):1738-1742,5.基金项目
国家自然科学基金（91019004，91229123）；国家中医临床研究基地“龙医团队”项目（LYTD-21，JDZX2012123） Supported by National Natural Science Foundation of China (91019004,91229123) （91019004,91229123）
