南方医科大学学报Issue(12)：1748-1752,5.DOI:10.3969/j.issn.1673-4254.2014.12.08
miR-92b对人胃癌细胞SGC7901迁移、粘附和侵袭的影响
Effect of miR-92b on migration, adhesion and invasion of human gastric cancer cell line SGC7901
摘要
Abstract
Objective To investigate the effect of miR-92b on the migration, adhesion and invasion of gastric cancer cell line SGC7901. Methods The miR-92b inhibitor and mimics were transiently transfected in SGC7901 cells. The changes in the migration, adhesion and invasion of the transfected cells were tested with wound healing assay, Transwell migration assay, matrigel adhesion and Transwell invasion assay. The cellular expression of E-cadherin, vimentin, Akt and p-Akt were analyzed by Western blotting. Results The migration, adhesion and invasion assays showed that transfection with the inhibitor of miR-92b obviously decreased the numbers of gastric cancer cells. The expression of E-cadherin, AKT, and pAKT increased and vimentin decreased significantly in the cells transfected with the inhibitor of miR-92b. Transfection with the mimics of miR-92b produced opposite effects in SGC7901 cells. Conclusion miR-92b promotes the migration, adhesion and invasion of human gastric cancer cell line SGC7901 by mediating epithelial-mesenchymal transition, and may accelerate tumor cell metastasis via signaling pathways other than PI3K/Akt pathway.关键词
miR-92b/胃癌/迁移/粘附/侵袭/上皮-间质转化Key words
miR-92b/gastric cancer/cell migration/cell adhesion/cell invasion/epithelial-mesenchymal transition引用本文复制引用
王卫华,王昌正,蒋一,吴本俨..miR-92b对人胃癌细胞SGC7901迁移、粘附和侵袭的影响[J].南方医科大学学报,2014,(12):1748-1752,5.基金项目
国家自然科学基金（81070296） Supported by National Natural Science Foundation of China (81070296) （81070296）
