南方医科大学学报Issue(1)：6-11,6.DOI:10.3969/j.issn.1673-4254.2015.01.02
晚期糖基化终产物受体在脂多糖介导小鼠肺微血管内皮细胞骨架变化中的作用
Role of RAGE in lipopolysaccharide-induced cytoskeletal changes in mouse pulmonary mi-crovascular endothelial cells
摘要
Abstract
Objective To investigate lipopolysaccharide (LPS)-induced changes of cytoskeletal filamentous actin in primary isolated pulmonary microvascular endothelial cells (PMVECs) from wild-type and RAGE knock-out mouse. Methods The lungs of wild-type and RAGE knock-out mice were digested with collagenase type I to obtain endothelial cells purified by anti-CD31-coupled magnetic beads. The PMVEC identified by factor VIII labeling were stimulated with LPS at different concentrations and the changes of filamentous actin were observed by confocal microscopy. Results The cultured primary cells showed typical endothelial cell phenotype as examined with factor VIII labeling. LPS stimulation caused rearrangement of the cytoskeletal filament F-actin in wild-type mouse PMVECs with stress fiber formation, but such changes were not obvious in RAGE knock-out mouse PMVECs. Conclusion Mouse PMVECs of a high purity can be obtained by immune magnetic beads. RAGE is involved in LPS-induced destruction of mouse PMVEC cytoskeletons.关键词
小鼠肺微血管内皮细胞/脂多糖/晚期糖基化终产物受体/细胞骨架Key words
mouse pulmonary microvascular endothelial cells/lipopolysaccharide/receptor, advanced glycation end products/cytoskeleton引用本文复制引用
周晓燕,张伟金,黄巧冰,郭晓华..晚期糖基化终产物受体在脂多糖介导小鼠肺微血管内皮细胞骨架变化中的作用[J].南方医科大学学报,2015,(1):6-11,6.基金项目
国家自然科学基金青年基金（31300950）；教育部博士点博士学科点专项科研基金联合资助课题新教师类（20124433120011）；广东省医学科学技术研究基金（B2010176）Supported by National Natural Science Foundation of China (31300950) （31300950）
