南方医科大学学报Issue(1)：29-33,5.DOI:10.3969/j.issn.1673-4254.2015.01.06
高糖激活WNT信号通路促进血管平滑肌细胞钙化
High glucose promotes vascular smooth muscle cell calcification by activating WNT signaling pathway
摘要
Abstract
Objective To investigate whether high glucose-induced vascular calcification is associated with WNT signaling pathway. Methods An in vitro model of human vascular smooth muscle cell (VSMC) calcification was induced by exposure of the cells to high glucose. The expressions of WNT signal molecules and bone-related proteins including Cbfa1, Osx, OCN and BMP2 were analyzed with qRT-PCR, and the cell calcification was assessed by alizarin red staining. The effect of Dkk1, a WNT signaling inhibitor, on high glucose-induced cell calcification was tested with alizarin red staining and calcium content analysis. Results High glucose activated WNT signaling pathway in human VSMCs by up-regulating the expressions of WNT signal molecules including Wnt3a, Wnt7a, Fzd4 and Wisp1 mRNA by 1.86, 1.68, 2.1, and 2.3 folds, respectively, and by promoting the phosphorylation ofβ-catenin (2.70±0.22, P<0.05), a key mediator of WNT signaling pathway. Inhibition of WNT signaling pathway by Dkk1 attenuated high glucose-induced VSMC calcification and down-regulated the expression of bone-related proteins Cbfa1, Osx, OCN, and BMP2 by (51 ± 9)%, (58 ± 11)%, (56 ± 10)%, and (62 ± 10)% (P<0.01). Conclusion WNT signaling pathway is involved in high glucose-induced VSMC calcification.关键词
血管钙化/WNT/高糖/β-catenin/血管平滑肌细胞Key words
vascular calcification/WNT/high glucose/β-catenin/vascular smooth muscle cells引用本文复制引用
颜建云,周芹,于汇民,侯梦琳,陆立鹤..高糖激活WNT信号通路促进血管平滑肌细胞钙化[J].南方医科大学学报,2015,(1):29-33,5.基金项目
国家自然科学基金（81000124，81470488）；中山大学高校基本科研业务费专项资金（11ykpy8）；教育部留学回国人员科研启动基金资助项目Supported by National Natural Science Foundation of China (81000124，81470488) （81000124，81470488）
