南方医科大学学报Issue(1)：93-97,5.DOI:10.3969/j.issn.1673-4254.2015.01.18
癌源性免疫球蛋白G全分子相互作用蛋白质的质谱鉴定及其功能分析
Functional analysis of cancer-derived immunoglobulin G whole molecule-interacting pro-teins identified by LC-MS/MS
摘要
Abstract
Objective To identify cancer-derived immunoglobulin G (IgG) whole molecule-interacting proteins to provide important clues for studying IgG biological functions. Methods HeLa cell lysate was immunoprecipitated with rabbit antihuman IgG whole molecule antibody and normal rabbit IgG. The immunocomplex underwent sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and was detected with silver staining. Three prominently enhanced bands were subjected to protein identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the MS data were analyzed with Swiss-Prot database. Cancer-derived IgG whole molecule-interacting proteins were screened and functionally annotated. Results and Conclusion We identified 6 potential cancer-derived IgG whole molecule-interacting proteins with co-immunoprecipitation combined with LC-MS/MS, which provides valuable clues for studying the function of cancer-derived IgG.关键词
癌/免疫球蛋白G/质谱分析Key words
cancer/immunoglobulin G/liquid chromatography-tandem mass spectrometry引用本文复制引用
王居平,陈韩英,彭慧..癌源性免疫球蛋白G全分子相互作用蛋白质的质谱鉴定及其功能分析[J].南方医科大学学报,2015,(1):93-97,5.基金项目
国家自然科学基金（30971150）；汕头大学医学院博士后科研启动基金Supported by National Natural Science Foundation of China (30971150) （30971150）
