南方医科大学学报Issue(2)：163-167,5.DOI:10.3969/j.issn.1673-4254.2015.02.02
肾组织干细胞向肾小管上皮细胞的诱导分化
Induced differentiation of rat kidney stem cells into renal tubular epithelial cells
摘要
Abstract
Objective To investigate the differentiation capability of kidney stem cells (KSCs) into renal tubular epithelial cells (RTECs). Methods KSCs isolated from the renal papilla of 4-week-old SD rats were co-cultured with hypoxia-exposed RTEC in induced medium (containing activin A, BMP-7, and retinoic acid) and renal epithelial cell growth medium (REGM) alternately. The KSCs cultured in MSC medium served as the control. The KSC differentiation rates in both groups were determined using flow cytometry, immunofluorescence assay and qRT-PCR. Results Flow cytometry showed a CK-18 positive rate of 6.5%in the control KSC group and of 44.2%in the induced group. Immunofluorescence assay detected the positivity for mature epithelial cell markers CK-18, E-cadherin, and ZO-1 in the induced cells. The results of qRT-PCR showed significantly increased expression of E-cadherin and AQP-1 mRNAs in the induced cells compared with the control cells (P<0.01). Conclusion Rat KSCs can be induced to differentiate into RTECs in vitro.关键词
肾组织干细胞/诱导分化/肾小管上皮细胞/共培养Key words
kidney stem cells/differentiation/renal tubular epithelial cell/co-culture引用本文复制引用
杨光,程庆砾,李春霖,杨勇,贾雅丽,王静雪,岳文,裴雪涛,刘洋..肾组织干细胞向肾小管上皮细胞的诱导分化[J].南方医科大学学报,2015,(2):163-167,5.基金项目
国家自然科学基金（81170312，30772296）；北京市自然科学基金（7122163）；解放军总医院扶持基金 （）
