南方医科大学学报Issue(2)：234-238,247,6.DOI:10.3969/j.issn.1673-4254.2015.02.15
基于mtDNA-COI基因序列分析我国中华按蚊种群的遗传结构
Analysis of population genetic structure of Anopheles sinensis based on mitochondrial DNA cytochrome oxidase subunit I gene fragment
摘要
Abstract
Objective To study the population genetic variation, genetic diversity and phylogenesis of Anopheles sinensis in China. Methods Anopheles sinensis samples collected from Shandong, Anhui, Jiangsu, Guizhou, and Yunnan Provinces and Guangxi Zhuang Autonomous Region with different geographical conditions between 2010 and 2012 were analyzed by mitochondrial DNA cytochrome oxidase subunit I (mtDNA-COI) gene amplification and sequencing. Bioedit 7.0 and DnaSP 5.0 software was used to compare the gene sequences and analyze the population genetic structure, respectively. Arlequin 3.1 was used to calculate the genetic distance and parameters of population differentiation. The relationship between the geographic and genetic distances was analyzed using IBD Web Service. PHYLIP 3.6 was used to construct the phylogenetic tree. Results PCR amplification and sequencing was performed successfully for 6 Anopheles sinensis populations containing 123 female mosquitoes. The length of mtDNA-COI gene fragment was 841 bp with an average A+T content of 71.2% and G+C content of 28.8%. High nucleotide diversity and genetic differentiation were observed among the Anopheles sinensis populations based on mtDNA-COI gene. Analysis of the molecular variance revealed a greater variation between populations than that within populations with isolation by distance between the populations. The Anopheles sinensis populations appeared to have undergone expansion, but the Yunnan population constituted an isolated branch in the phylogenetic tree. Conclusion mtDNA-COI can serve as the molecular marker to analyze population genetic variation and phylogenesis of Anopheles sinensis. The Yunnan population shows a phylogenetic difference from the other populations analyzed in this study.关键词
中华按蚊/mtDNA-COI/种群遗传Key words
Anopheles sinensis/mtDNA-COI/population genetic引用本文复制引用
常雪莲,钟代斌,李小聪,黄亚铭,朱国鼎,魏星,夏惠,陈晓光,方强..基于mtDNA-COI基因序列分析我国中华按蚊种群的遗传结构[J].南方医科大学学报,2015,(2):234-238,247,6.基金项目
美国国立卫生研究院NIH项目(D43TW009527-01)；广东省普通高校新发传染病防治重点实验室开放基金项目(KLB09007)；安徽省自然科学基金(1308085MH160)；蚌埠医学院自然科学基金重点项目 （D43TW009527-01）
