南方医科大学学报Issue(2)：281-283,3.DOI:10.3969/j.issn.1673-4254.2015.02.26
慢病毒介导靶向survivin基因的shRNA抑制子宫内膜在裸鼠腹腔种植生长
Lentiviral vector-mediated short hairpin RNA targeting survivin inhibits abdominal growth of human endometrium xenograft in nude mice
摘要
Abstract
Objective To investigate the inhibitory effect of lentiviral vector-mediated short hairpin RNA targeting survivin (LV-survivin shRNA) on the growth of human endometrium xenograft in the abdominal cavity of nude mice. Methods The endometrium xenografts from 8 women with endometriosis were injected into the peritoneal cavities of 45 nude mice. The mice were then randomly assigned to receive intraperitoneal injection of LV-survivin shRNA, pGCL-NC-GFP (negative control) or PBS (blank control). Two weeks later, the number and morphometry of endometriotic lesions were quantified and the expression of survivin protein were detected by immunohistochemistry. Results The formation of endometriotic lesions was significantly suppressed in mice receiving LV-survivin shRNA injection as compared with those in the two control groups (P<0.001). The mice in LV-survivin-shRNA group showed significantly down-regulated expression levels of survivin protein compared with those in the negative and blank control groups, presenting also necrosis in the endometriosis-like lesions in microscopic observation. Conclusion Lentiviral vector-mediated shRNA can effectively inhibit the expression of survivin in human endometrium xengrafts and suppress the formation and growth of endometriotic lesions in the abdominal cavities of nude mice.关键词
生存素/短发夹状RNA/子宫内膜异位症/慢病毒/裸鼠Key words
survivin/short hairpin RNA/endometriosis/lentivirus/nude mice引用本文复制引用
彭冬先,何援利..慢病毒介导靶向survivin基因的shRNA抑制子宫内膜在裸鼠腹腔种植生长[J].南方医科大学学报,2015,(2):281-283,3.基金项目
广东省科技计划项目 （）
