南方医科大学学报Issue(3)：338-342,5.DOI:10.3969/j.issn.1673-4254.2015.03.05
miR-223在肾透明细胞癌中的表达与功能
Expression of miR-223 in clear cell renal cell carcinoma and its significance
摘要
Abstract
Objective To investigate the expression of miR-223 in clear cell renal cell carcinoma (ccRcc) and its clinical implications. Methods Quantitative real-time PCR was employed to detect the levels of miR- 223 expression in ccRcc, pair-matched adjacent normal tissues and different renal cancer cell lines. Transwell migration essay and wound healing essay were used to evaluate the invasion and migration of renal cancer 786-O cells transfected with miR-223 mimics. MTT essay was used to measure the cell proliferation, and the cell cycle changes following the transfection were analyzed with flow cytometry. Results Compared with the normal tissues, the cancer samples showed up-regulated miR-223 expression, which was associated with tumor size. In 786-O cell cultures, transfection with miR-223 mimics significantly enhanced cell migration (P<0.0001) and growth (P=0.006) and induced G1 cell cycle arrest. Conclusion miR-223 promotes renal cancer cell migration and proliferation and may serve as a potential therapeutic target for ccRcc.关键词
miR-223/肾透明细胞癌/迁移/增殖Key words
miR-223/clear cell renal cell carcinoma/migration/proliferation引用本文复制引用
牛少曦,张旭,马鑫,张瑜,巩会杰,高宇,李新涛,沈东来,王雷,姚远新..miR-223在肾透明细胞癌中的表达与功能[J].南方医科大学学报,2015,(3):338-342,5.基金项目
国家高技术研究发展计划（863计划）（2014AA020600，2012AA02101）Supported by National High Technology Research and Development Program of China (2014AA020600,2012AA02101) （2014AA020600,2012AA02101）
