南方医科大学学报Issue(3)：450-454,5.DOI:10.3969/j.issn.1673-4254.2015.03.28
miR-200a在结直肠癌细胞系中的表达及其对高转移细胞系Lovo的影响
Expression of miR-200a in colorectal carcinoma cell lines and its effect on LoVo cells
吴共发 1赵海燕 2何楠 3韩慧霞4
作者信息
- 1. 增城市人民医院病理科，广东 增城 511300
- 2. 深圳市龙岗中心医院病理科，广东 深圳 518116
- 3. 郑州市儿童医院病理科，河南 郑州450053
- 4. 南方医科大学基础医学院病理学系，广东 广州 510515
- 折叠
摘要
Abstract
Objective To detect miR-200a expression in human colorectal carcinnoma (CRC) cell lines and explore the role of miR-200a in regulating the biological behavior of CRC cells. Methods Real-time quantitative RT-PCR (qRT-PCR) was used to detect miR-200a expression levels in 6 CRC cell lines (HCT116, HT29, LS174T, SW480, SW620 and LoVo). miR-200a mimics were transiently transfected into LoVo, and the changes in cell proliferation, apoptosis, migration, and cell-cell adhesion were assessed using CCK-8 assay, TUNEL assay, transwell migration assay, and homogenous adhesion experiment, respectively. Results The expression of miR-200a was down-regulated in the 6 CRC cell lines, among which the highly metastatic LoVo cell line showed the lowest expression and the tumorigenic but non-metastatic CRC cell line HCT116 had the highest expression. Overexpression of miR-200a depressed cell proliferation and migration but promoted cell apoptosis and cell-cell adhesion in LoVo cells. Conclusion miR-200a plays a role in regulating the invasiveness and metastasis of CRC, and overexpression of miR-200a causes a significant reduction of cell proliferation and migration and promotes apoptosis and cell-cell adhesion in LoVo cells.关键词
miR-200a/结肠癌/增殖/迁移/凋亡/粘附Key words
miR-2000a/colorectal carcinnoma/proliferation/migration/apoptosis/adhesion引用本文复制引用
吴共发,赵海燕,何楠,韩慧霞..miR-200a在结直肠癌细胞系中的表达及其对高转移细胞系Lovo的影响[J].南方医科大学学报,2015,(3):450-454,5.
