南方医科大学学报Issue(5)：619-624,6.DOI:10.3969/j.issn.1673-4254.2015.05.01
灵芝酸A对人骨肉瘤细胞增殖、凋亡和迁移的影响
Ganoderic acid A suppresses proliferation and invasion and induces apoptosis in human osteosarcoma cells
摘要
Abstract
Objective To investigate the effect of ganoderic acid A (GA-A) on the biological behaviors of human osteosarcoma cells in vitro. Methods MG63 and HOS cells were treated with 0.1, 0.25, and 0.5 mmol/L GA-A, and the changes in cell proliferation, apoptosis and migration were evaluated using MTT assay, flow cytometry, and Transwell assay, respectively. The expressions of STAT3, p38, and NF-κB1 in the cells were analyzed by Western blotting. Results GA-A effectively inhibited the proliferation of human osteosarcoma HOS and MG-63 cells in a dose-dependent manner, and induced obvious cell apoptosis in both cells. Treatment with 0.5 mmol/L GA-A also resulted in significant inhibition of the invasion of both cells. The results of Western blotting showed that GA-A down-regulated the expression level of phosphorylated STAT3 and increased the phosphorylation level of p38 and NF-κB1 expression in both cells. Conclusion GA-A can induce proliferation inhibition, apoptosis and suppression of invasion in human osteosarcoma HOS and MG-63 cells.关键词
灵芝酸A/人骨肉瘤/增殖/凋亡Key words
ganoderic acid A/human osteosarcoma cells/proliferation/apoptosis/invasion引用本文复制引用
邵建立,李志忠,焦根龙,孙国栋,周志刚..灵芝酸A对人骨肉瘤细胞增殖、凋亡和迁移的影响[J].南方医科大学学报,2015,(5):619-624,6.基金项目
国家自然科学基金（30973127）；广东省中医药局基金 （）
