南方医科大学学报Issue(5)：720-723,4.DOI:10.3969/j.issn.1673-4254.2015.05.19
广东及海南蝙蝠乙型脑炎病毒血清抗体的检测
Detection of serum antibodies against Japanese encephalitis virus in bats in Hainan and Guangdong Provinces of China
摘要
Abstract
Objective To investigate the prevalence of serum antibodies against Japanese encephalitis virus (JEV) in bats. Methods Blood samples from the heart were obtained from bats captured in Guangdong and Hainan Provinces in 2013. The anti-JEV antibodies in bat sera were tested using indirect ELISA and virus neutralization test. Results A total of 201 bat serum samples were tested, in which the total positivity rate of anti-JEV antibodies was 46.27%(93/201). The positive rate of anti-JEV antibodies in bats from Hainan and Guangdong Provinces was 88.89% (48/54) and 30.61% (45/147), respectively. All the samples from Rousettus leschenaultia, Miniopterus schreibersii, Pipistrellus abramus, and Rhinolophus macrotis were positive for anti-JEV antibodies, and up to 95.56%(43/45) of the samples from Miniopterus schreibersii (from Hainan Province) yielded positive results. Of the 28 samples with positive results by indirect ELISA, 15 showed positive results in virus neutralization test (53.57%) with neutralization antibody titers ranging from 1∶10 to 1∶28.22. Conclusion Bats from different regions and of different species can be naturally infected with JEV and have a high prevalence of anti-JEV antibodies in their sera. The role of bats in the natural cycle of JEV awaits further study.关键词
蝙蝠/乙型脑炎病毒抗体/酶联免疫吸附试验/病毒中和试验Key words
bats/Japanese encephalitis virus antibody/enzyme-linked immunosorbent assay/virus neutralization test引用本文复制引用
蒋丽娜,王祉蕴,陈清,陈少威,郑雪燕,马淑娟,周军华,张琼花,李杏,熊益权,钟雪珊..广东及海南蝙蝠乙型脑炎病毒血清抗体的检测[J].南方医科大学学报,2015,(5):720-723,4.基金项目
国家自然科学基金（30972525） Supported by National Natural Science Foundation of China (30972525) （30972525）
