南方医科大学学报Issue(7)：1024-1027,1033,5.DOI:10.3969/j.issn.1673-4254.2015.07.17
慢病毒介导shRNA靶向ZNF217抑制胶质瘤细胞生长、迁移和侵袭
Lentivirus-mediated shRNA targeting ZNF217 suppresses cell growth, migration, and invasion of glioma cells in vitro
摘要
Abstract
Objective To explore the role of ZNF217 in regulating cell proliferation, migration and invasion in glioma cells. Methods A lentivirus-mediated shRNA-ZNF217 vector was infected into glioma U251 cells, and the interference efficiency was examined by Western blotting. MTT assay, flow cytometry, Transwell assay, and Boyden chamber assay were used to analyze the changes in cell proliferation, migration and invasion. Western blotting was used to detect the changes in ZNF217-related genes in the cells. Results shRNA-ZNF217 transfection significantly inhibited the expression of ZNF217 in U251 cells and suppressed the cell migration, invasion, growth, and cell cycle transition. ZNF217 knockdown downregulated the expression of pPI3, pAKT, C-Myc, and the mesenchyme biomarker N-cadherin, and stimulated the expression of the epithelium biomarker E-cadherin. Conclusion ZNF217 promotes cell migration, invasion, and growth by activating PI3K/AKT signal to upregulate C-Myc and by modulating the genes associated with epithelial-mesenchymal transition in glioma cells.关键词
ZNF217/胶质瘤/shRNAKey words
ZNF217/glioma/short-hairpin RNA引用本文复制引用
罗起胜,栗学玉,黄海能,邓元央,黄华东,符黄德,罗琨祥,李传玉,覃成箭,韦展亮..慢病毒介导shRNA靶向ZNF217抑制胶质瘤细胞生长、迁移和侵袭[J].南方医科大学学报,2015,(7):1024-1027,1033,5.基金项目
广西自然科学基金（2014GXNSFBA118143）；广西高校科学技术研究项目 （）
