福建林学院学报Issue(4)：289-296,8.
大苞鞘石斛胚组织培养及植株再生研究
In vitro tissue culturea nd plant regeneration from mature embryos of Dendrobium warkianum
摘要
Abstract
The tissue culture and plant regeneration of Dendrobium wardianum were studied by using embryos as material.The embryos were cultivated in 1/2 MS medium using different plant hormones and additives with different concentrations by orthogonal design test to examine those factors which affect the germination and regeneration, the perfect mediums for different growth periods were screened, and the rapid propagation system of D.wardianum.The results indicated as follows.The optimum medium for the embryo germination and enlargement was 1/2 MS +IBA 1.0 mg· L-1 +6-BA 1.0 mg· L-1 +NAA 2.0 mg· L-1 +banana mud 50 g· L-1 with the germination rate of 91.29%.The optimum medium for differentiation was 1/2 MS+IBA 0.5 mg· L-1 +6-BA 1.0 mg· L-1 +NAA 0.5 mg· L-1 +banana mud 50 g· L-1 .The optimum medium for dry matter accumulation was 1/2 MS+IBA 2.0 mg· L-1 +6-BA 2.0 mg· L-1 +NAA 0.5 mg· L-1 +banana mud 200 g· L-1 .The optimum medium for proliferation of cluster buds was 1/2 MS +IBA 0.5 mg· L-1 +6-BA 1.0 mg· L-1 +NAA 0.5 mg· L-1 +banana mud 100 g· L-1 with the average proliferation rate of 63.3 .The optimum medium for rooting was 1/2MS +IBA 0.5 mg· L-1 +6-BA 3.0 mg· L-1 +NAA 0.2 mg· L-1+banana mud 50 ·g L-1 +mashed potato 50 g· L-1 .关键词
大苞鞘石斛/胚培养/增殖/生根Key words
Dendronium warkianum/embryo culture/proliferation/rooting分类
农业科技引用本文复制引用
卓孝康,兰思仁,彭东辉,吴沙沙,江鸣涛,张林瀛..大苞鞘石斛胚组织培养及植株再生研究[J].福建林学院学报,2014,(4):289-296,8.基金项目
林业公益性行业科研专项（201204604）；福建省财政基金项目（2012）。 （）
