森林与环境学报Issue(1)：60-66,7.DOI:10.13324/j.cnki.jfcf.2015.01.010
基于慈竹转录组 MYB 基因的克隆及胁迫诱导表达
Cloning of MYB gene based on Bambusa emeiensis transcriptome and their induced expression under stresses
摘要
Abstract
According to the transcriptome data of Bambusa emeiensis Chia et H.L.Fung‘Viridiflava ’ shoots, two MYB genes designated as BeMYB1 and BeMYB2 were cloned and their bioinformatics were analyzed .The mechanism of their response to abscisic acid ( ABA ) , sodium chloride ( NaCl ) and polyethylene glycol 6000 ( PEG 6000 ) stresses was explored .The bioinformatics analysis results showed that BeMYB1 and BeMYB2 genes encoded 632 and 338 amino acids respectively .BeMYB1 and wheat TaMYB48 proteins were clustered into the same branch , which were MYB-related proteins with two motifs .While BeMYB2 protein belonged to R2R3-MYB protein with three motifs , and it was aggregated into one branch with bamboo PeMYB 2 and rice OsMYB18 proteins.The stress induced expression analysis results displayed that BeMYB1 and BeMYB2 genes responded to ABA , NaCl and PEG 6000, but the response ability of BeMYB2 was higher than BeMYB1.Hence, it is indicated that BeMYB1 and BeMYB2 genes played an important role in the responses to abiotic stresses .关键词
慈竹/MYB基因/克隆/生物信息学分析/诱导表达Key words
Bambusa emeiensis Chia et H.L.Fung‘Viridiflava’/MYB gene/cloning/bioinformatics analysis/induced expression分类
农业科技引用本文复制引用
杨传凤,曹颖,胡尚连,徐刚,黄艳,龙治坚..基于慈竹转录组 MYB 基因的克隆及胁迫诱导表达[J].森林与环境学报,2015,(1):60-66,7.基金项目
国家自然科学基金项目（31400257，31400333）；四川省应用基础研究基金项目（2013JY0182）；四川省生物质资源利用与改性工程技术研究中心基金项目（12zxsk07，13zxsk01）；西南科技大学研究生创新基金项目（14ycxjj0079）。 （）
