福建农业学报Issue(4)：301-305,5.
种番鸭源禽坦布苏病毒分离鉴定及基因组序列分析
Identification and Genome Sequence Analysis of Tembusu Virus Isolated From Breed Muscovy Duck
摘要
Abstract
Nowadays ,it is still unknow whether the avian tembusu virus could infect breed Muscovy duck .For the first time ,we obtained a viral isolate (named WYZLJ-1359) from ovary of breed Muscovy duck with decreasing egg-laying rate ,and confirmed the infectious agent as avian tembusu virus (ATV) using RT-PCR .Results of sequence comparison with viral genome showed that the main molecular characteristics of genome sequence of WYZLJ-1359 strain were in accordance with those of other viruses isolated from other domestic poultry since 2010 ,and shared no less than 98.4% nucleotide sequence identity with each other .Phylogenetic analysis showed that all ATVs isolated since 2010 shared closed relationship with each other .And the genetic distance (GD) among these viruses were all no more than 1.8% ,which was far less than the GD between WYZLJ-1359 strain and those viruses reported before 2000. These results indicated that although the host spectrum of avian tembusu virus continued to increase ,the virus showed genetic stability during the process of spread among different poultry species without deletion or insertion in viral genome .关键词
坦布苏病毒/种番鸭/分离鉴定/基因组Key words
tembusu virus/breed Muscovy duck/isolation and identification/genome分类
农业科技引用本文复制引用
傅光华,陈红梅,黄瑜,万春和,傅秋玲,程龙飞,施少华,林建生..种番鸭源禽坦布苏病毒分离鉴定及基因组序列分析[J].福建农业学报,2014,(4):301-305,5.基金项目
国家自然科学基金项目（31201936）；现代农业产业技术体系建设专项（CARS-43）；福建省种业创新与产业化工程项目（2011FJZY-9）；福建省科技计划项目-省属公益类科研院所基本科研专项 （）
