福建农业学报Issue(4)：310-313,4.
新型鸭呼肠孤病毒σC基因原核表达及其抗原性的初步研究
Prokaryotic Expression and Initial Antigenicity Analysis of Novel Duck Reovirus σC Protein
摘要
Abstract
To obtain σC protein of Novel Duck Reovirus (NDRV ) from prokaryotic expression system and initial identification of its antigenicity ,theσC gene of NDRV was amplified by RT-PCR from NDRV NP03 strain using the specific primers which were designed according to the published cDNA sequence .Subsequently ,the target gene was cloned into pET-30a (+ ) prokaryotic expression vector to construct the recombinant plasmid pET-NDRV-σC , which was transformed into BL21 (DE3) and the expression of the σC gene was induced (1.0 mmol · L -1 IPTG , 37℃) .The fusion protein was identified by SDS-PAGE and purified .The antigenicity of the purified protein was characterized by Western blotting .TheσC gene was obtained with an identical sequence of 966 bp to that retrieved in GenBank .The prokaryotic expression vector for σC gene was successfully constructed as confirmed by enzyme digestion and DNA sequencing .The result of SDS-PAGE showed that the fusion protein had a relative molecular weight of 41.3 ku . Moreover , the σC protein obtained form prokaryotic expression system possessed good immunoreactivity which was showed by western blotting .Together ,our work laid foundation for further study of NDRV σC protein .关键词
新型鸭呼肠孤病毒/σC蛋白/原核表达/Western blotting/抗原性Key words
Novel Duck Reovirus/σC protein/Prokaryotic expression/Western blotting/Antigenicity分类
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王锦祥,程晓霞,陈少莺,陈仕龙,林锋强,王劭,李兆龙..新型鸭呼肠孤病毒σC基因原核表达及其抗原性的初步研究[J].福建农业学报,2014,(4):310-313,4.基金项目
国家高技术研究发展计划（863计划）项目（2011AA10A209）；福建省科技计划项目---省属公益类科研院所基本科研专项 （）
