垂花蕙兰丛生芽途径的组织培养技术研究OA北大核心CSTPCD
In Vitro Micropropagation of Pendent Cymbidium with Clustered Shoots
以侧芽为外植体,对垂花蕙兰丛生芽途径的组织培养进行研究,结果表明,采用72%乙醇、0.1%升汞和15%次氯酸钠对侧芽进行3次消毒,存活率高达56%,尤其是在春季外植体消毒效果佳;在丛生芽诱导的过程中,6-BA浓度为4.0 mg · L -1时,丛芽诱导率可达100%且丛芽分化较多;在丛生芽增殖过程中,6-BA是影响垂花蕙兰丛生芽增殖的重要因素,6-BA 4.0 mg · L -1处理增殖系数为3.4,丛生芽较健壮,适合丛生芽增殖;培养基1/2MS…查看全部>>
Lateral buds were used as the explants for the study on tissue culture of pendent Cymbidium .It showed that by using 72% ethanol ,0.1% mercuric chloride and 15% sodium hypochlorite to disinfect the lateral buds three times ,the bud survival rate could be as high as 56% .The disinfection was especially effective in springtime .The induction rate of the clustered shoots was up to 100% with numerous differentiated buds when 6-BA was applied at the rate of 4.0 m…查看全部>>
周辉明;陈燕;林辉锋;尚伟;王雪凤
福建省三明市农业科学研究院,福建 沙县 365051福建省三明市农业局,福建 三明 365000福建省三明市农业科学研究院,福建 沙县 365051福建省三明市农业科学研究院,福建 沙县 365051福建省三明市农业科学研究院,福建 沙县 365051
农业科技
垂花蕙兰丛生芽组织培养
Pendent Cymbidiumclustered shootstissue culture
《福建农业学报》 2014 (4)
339-344,6
福建省农业科学院“海西农作物品种引种中心区域引种圃建设”项目
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