福建农业学报Issue(5)：461-464,4.
细叶石仙桃无菌播种与快速繁殖技术研究初报
A Preliminary Report on Aseptic Seeding and Rapid Propagation of Pholidota cantonensis Rolfe
摘要
Abstract
To alleviate a seed shortage on Pholidota cantonensis Rolfe ,artificial culturing with medium optimization was investigated by using the plant tissue culture technology .The results showed that MS+20 g · L -1 sucrose+8 g · L -1 agar was an appropriate medium that allowed a germination rate above 70% .MS+50 g · L -1 potato+20 g · L -1 sucrose+8 g · L -1 agar was desirable for the plantlet′s protocorm differentiation .Low concentrations of 6-BA and NAA in medium was better for bud protocorm proliferation and differentiation .The medium formulated with 1/2 MS+0.5 mg · L -1 NAA+20 g · L -1 sucrose+ 8 g · L -1 agar was optimal for rooting ,as a rate of 100%was achieved .By using the combination of the above-mentioned media at respective stages ,the plantlets of P . cantonensis Rolfe could be rapidly obtained from seeds .关键词
细叶石仙桃/种子/无菌播种/快速繁殖Key words
Pholidota cantonensis Rolfe/seed/aseptic seeding/rapid propagation分类
医药卫生引用本文复制引用
刘保财,黄颖桢,赵云青,陈菁瑛..细叶石仙桃无菌播种与快速繁殖技术研究初报[J].福建农业学报,2014,(5):461-464,4.基金项目
福建省农业科学院导师制青年基金项目 （）
