福建农业学报Issue(7)：641-645,5.
水稻泛素连接酶基因OsHUB2实时荧光定量PCR方法的建立
RT-PCR Analysis for Ubiquitin Ligase,OsHUB2,from Rice
摘要
Abstract
(1 .RiceResearch Institute ,FujianAcademy of Agricultural Sciences ,Fuzhou,Fujian 350019 ,China;2 . Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China ,Ministry of Agriculture ,P.R China/Fuzhou branch ,National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding ,Fuzhou , Fujian 350003 ,China;3 . Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology ,P.R.China , Fuzhou ,Fujian 350003 ,China;4 . National Rice Engineering Laboratory of China ,Fuzhou , Fujian 350003 ,China;5 . South China Bases of National Key Laboratory of Hybrid Rice for China ,Fuzhou ,Fujian 350003 ,China;6.Fruit Research Institute ,Fujian Academy of Agricultural Sciences ,Fuzhou ,Fujian 350013 ,China)and OsHUB2-F3R3 ,were designed using the Primer Premier 5.0 according to the sequence of the enzyme from GeneBank .Subsequently ,RT-PCR was conducted to select the best primer pairs with a specific and single amplification product . The results showed OsHUB2-F3R3 to be desirable on both and functions . The analytical procedure was thus considered of use in future studies on the rice ubiquitin ligase .关键词
水稻/泛素连接酶基因/荧光定量PCRKey words
rice/ubiquitin ligase Os H UB2/RT-PCR (qRT-PCR)分类
农业科技引用本文复制引用
何炜,朱永生,连玲,周平,蔡秋华,王颖姮,谢鸿光,张建福,谢华安..水稻泛素连接酶基因OsHUB2实时荧光定量PCR方法的建立[J].福建农业学报,2014,(7):641-645,5.基金项目
国家“863”计划项目（2014AA10A603、2014AA10A604）；福建省自然科学基金项目 （）
