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菌株TLB15发酵培养基的优化及其发酵液的杀虫活性

赵晓燕 吴晓青 赵忠娟 陈泉 李哲 李纪顺 杨合同

福建农业学报Issue(7):669-672,4.
福建农业学报Issue(7):669-672,4.

菌株TLB15发酵培养基的优化及其发酵液的杀虫活性

Optimum Fermentation Medium for Insecticidal Active Substances from Strain TLB 15

赵晓燕 1吴晓青 1赵忠娟 1陈泉 1李哲 1李纪顺 1杨合同1

作者信息

  • 1. 山东省科学院中日友好生物技术研究中心/山东省应用微生物重点实验室,山东 济南 250014
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摘要

Abstract

The optimum fermentation culture medium of strain TLB15 was modified through an orthogonal experiment L18 (37 ) . The results showed that the optimum fermentation culture medium contained 3.0% corm meal ,2.0% soybean powder , 1.0% sugar , 0.1% ammonium sulfate , 0.3% potassium hydrogen phosphate trihydrate ,0.2% potassium dihydrogen phosphate ,and 0.04% magnesium sulfate heptahydrate .The number of viable cells was 4.52 × 109 cfu · mL -1 and the rate of spore formation was 90% after 48hs′fermentation .The 72-h adjusted mortality of TLB15 strain fermentation liquid on the larvae of Plutella xylostella and Spodoptera exigua w ere 66.93% and 69.07% ,respectively .

关键词

发酵/培养基优化/正交设计/菌株T LB15/杀虫活性

Key words

fermentation/medium optimization of/orthogonal experiment/strain TLB15

分类

农业科技

引用本文复制引用

赵晓燕,吴晓青,赵忠娟,陈泉,李哲,李纪顺,杨合同..菌株TLB15发酵培养基的优化及其发酵液的杀虫活性[J].福建农业学报,2014,(7):669-672,4.

基金项目

山东省科技攻关项目 ()

福建农业学报

OA北大核心CSTPCD

1008-0384

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