福建农业学报Issue(10)：935-938,4.
鸭坦布苏病毒囊膜 E蛋白的原核表达
Prokaryotic Expression of E Protein from Duck Tembusu Virus
摘要
Abstract
The entire E gene from the duck Tembusu virus (strain WR)was amplified by RT-PCR,and cloned into thepEASY-T3 vector.The recombinant plasmids carrying the target fragment were digested with Bam HI and Xho I,andcloned into the pET-32a vector to construct recombinant plasmid to be named pET-E.pET-E was subsequently transformedinto Escherichia coli BL21 (DE3 ).The fusion protein was expressed by IPTG induction,and presented mainly asinclusion bodies.The positive western blotting result suggested a strong reactinogenicity of the protein.关键词
鸭坦布苏病毒/E基因/原核表达Key words
duck tembusu virus/E gene/prokaryotic expression分类
农业科技引用本文复制引用
施少华,程龙飞,傅光华,陈红梅,万春和,傅秋玲,林建生,黄瑜..鸭坦布苏病毒囊膜 E蛋白的原核表达[J].福建农业学报,2014,(10):935-938,4.基金项目
福建省科技计划项目---省属公益类科研院所基本科研专项（2011R1025-8）；现代农业产业技术体系建设专项（CARS-43）；福建省种业创新与产业化工程建设项目 （）
