福建农业学报Issue(10)：970-975,6.
福建15个茶树品种SSR 遗传差异分析与指纹图谱建立
Genetic Variances and DNA Fingerprints of 1 5 Teas in Fujian
摘要
Abstract
Identification and preservation of tea cultivars plays a crucial role in the production and development of thetea industry in China.This paper presents the genetic variances and molecular fingerprints of 1 0 tea cultivarsselected and cultivated by our research team and 5 control cultivars in Fuj ian by using SSR molecular markers.Itaimed to further clarify the genetic differences of these cultivars providing a basis for identification of newlyimproved tea cultivars and protection of intellectual property rights.In the course,6 core primer pairs with highpolymorphism were selected from the 1 05 published pairs.The genetic similarity coefficient of the 1 5 tested cultivarsvaried greatly,ranging from 0.3 5 to 0.9 1. It generally reached above average (≥0.6 5)with high at 0.80-0.9 1 forthe 8 tea cultivars and 4 control cultivars from South and North Fuj ian among the tested varieties.Consequently,there was little or no genetic differences among the cultivars.Applying the cluster analysis,all tested materialscould be divided into 8 groups at the similarity coefficient of 0.7 7 ,and 6 groups in the similarity coefficient of 0.6 6.The molecular fingerprints of the tested cultivars were established using the primer pairs combination of W08,D07and L1 1. The characteristic stripes obtained were between 2 1 0-6 30 bp,which could conceivably suffice successfulidentifications of individual cultivars.关键词
茶树/选育品种/SSR/遗传差异/指纹图谱Key words
tea[Camelliasinensis(L.)O.Kuntze]/cultivars/SSR/geneticvariance/fingerprint分类
农业科技引用本文复制引用
王让剑,杨军,孔祥瑞,郭吉春..福建15个茶树品种SSR 遗传差异分析与指纹图谱建立[J].福建农业学报,2014,(10):970-975,6.基金项目
福建省农业科学院科技创新团队建设项目（CXTD-1-1302）；安徽农业大学农业部茶树生物学与茶叶加工重点实验室开放基金项目 （）
