福建农业学报Issue(1)：60-64,5.
脂肪酸提取方法对芽胞杆菌种类脂肪酸测定结果的影响
The Influence of the Fatty Acid Extraction Method on the Fatty Acid Determination of the Aacillus Species
摘要
Abstract
Fatty acids were important components of cell membrane of bacteria,and were subjected to the control of genetic material on cell membrane. The components and contents of fatty acids of Bacillus species were highly conserved and stable. Differences in chain length,position of double bond and binding of functional group ,made fatty acids very useful as taxonomic markers. In bacterial culture and fatty acid extraction stages,the experiment results showed that the identification of Bacillus′s fatty acids was affected by saponification time and fatty acid extraction reagent dose. Using type species as object,effects of different detection conditions on fatty acid results of the genus Bacillus were investigated. The optimum conditions for the detection were saponification time of 25 to 30 min,saponification reagent dosage of 1. 0 mL,methylation reagent dosage of 1. 5 mL.关键词
芽胞杆菌/脂肪酸/提取方法Key words
Bacillus/fatty acids/extract method分类
农业科技引用本文复制引用
刘国红,刘波,唐建阳,车建美,朱育菁,陈峥..脂肪酸提取方法对芽胞杆菌种类脂肪酸测定结果的影响[J].福建农业学报,2015,(1):60-64,5.基金项目
国家自然科学基金项目（31370059）；农业部948项目（2011-G25）；科技部国际合作项目 （）
