福建农业学报Issue(1)：75-79,5.
超微粉碎对大杯蕈菇柄多糖溶出效果的影响
Effect of Ultra-fine Grinding on Polysaccharide Dissolubility of Clitocybe maxima Stipe
摘要
Abstract
To increase extraction rate in shorted extraction time,microwave-assisted technology was used for extracting polysaccharides of Clitocybe maxima stipe ground by particle size of 20 mesh vs 300 mesh. The crude polysaccharide was extracted from coarse and ultra-fine powder with the solvent water and precipitated with ethanol, then the content was detected using the phenol-sulfuric acid method. The effect on polysaccharide dissolubility was studied for the superfine grinding technology. The results showed that the best extraction parameters for ultra-fine powder were:extraction time 2 h,extraction temperature 80℃,the ratio of material to solvent 1∶40 ,extracting twice,and obtained polysaccharides 8. 93%.The extraction parameters for coarse powder were:extraction time 4 h,extraction temperature 90℃,the ratio of material to solvent 1∶40,extracting once and polysaccharide yield 5. 86%.Comparing with coarse powder,extracting polysaccharides with ultra-fine powder shortened extraction time for 50%,increased polysaccharide yield by 3. 07 percentage points,and improved the purity of polysaccharides by 12. 14 percentage points.It was indicated that ultra-fine grinding technology has improved the extraction efficiency of polysaccharide for Clitocybe Maxima stipe.关键词
大杯蕈菇柄/超微粉碎/多糖/提取Key words
Clitocybe maxima stem/ultra-fine grinding/polysaccharides/extraction分类
轻工纺织引用本文复制引用
赖谱富,方汝涛,陈君琛,杨艺龙..超微粉碎对大杯蕈菇柄多糖溶出效果的影响[J].福建农业学报,2015,(1):75-79,5.基金项目
福建省农业科学院导师制青年基金项目（2012DG-3）；国家公益性行业（农业）科研专项（201303080）；国家科技支撑计划项目（2012BAD14B15）；福建省科技重大专项 （）
