福建农业学报Issue(3)：253-257,5.
罗汉果组培苗生产体系的建立
Establishment of Tissue Culture System for Siraitia grosvenorii
摘要
Abstract
The stem segment of Siraitia grosvenorii was used as the material for establishing of tissue culture system with micropropogation.The results showed that the medium of MS + 6-BA 1.0 mg·L-1 + NAA 0.10 mg·L-1+ sucrose 30 g·L-1 + agar 6.0 g·L-1 was suitable for inducing axillary bud with induction rate of 40.0%. Single nodes cutting and accelerating for axillary buds growth were applied in subculture and optimal medium was 2/3 in modified MS + IBA 0.5 mg · L-1 + GA3 0.3 mg · L-1 + sucrose 40 g · L-1 + agar 6.0 g · L-1 ,with proliferation coefficient 4.0. The optimal rooting medium was 1/2 in modified MS + IBA 0.5 mg·L-1 + sucrose 2.0% + AC 0.1 % + agar 6.0 g·L-1 ,with rooting rate of 94%.The suitable transplanting medium was peat soil + pearlite in the ratio of 10∶2,with survival rate for 97.8%.关键词
罗汉果/茎段/组织培养/快速繁殖Key words
Siraitia grosvenorii Swingle C/stem/tissue culture分类
农业科技引用本文复制引用
陈汉鑫..罗汉果组培苗生产体系的建立[J].福建农业学报,2015,(3):253-257,5.基金项目
漳州市科技计划项目 （）
