福建农业学报Issue(5)：430-434,5.
绵羊肺炎支原体 FJ01-CL 株的分离和鉴定
Isolation and Identification of Mycoplasma ovipneumoniae FJ01-CL
摘要
Abstract
To confirm a suspected contagious pleuropneumonia case at a sheep farm in Fujian,the pathogen from the diseased sheep lung tissue was isolated and purified on a mycoplasma medium.It was subsequently identified by using a biochemical test and specificity polymerase chain reaction (PCR).There were " mulberries,no center umbilical"colonies on the petri dishes observed in the laboratory.The isolated FJ01-CL could ferment glucose,but could not hydrolyze arginine,nor decompose urea.It was positive on the Meilan reduction reaction,the 4-azole-nitrogen-chloride reduction reaction,the blood adsorption test and the cholesterol test.The PCR amplification of the sheep mycoplasma pneumoniae showed a specific band of 361 bp.Compared with 8 sequence strains from the Genbank,the sequence analysis indicated that the isolated FJ01-CL exhibited 98.1% homology with the reference strain,Y-98,and 98.7% with the local strains,MoGH3-3.The results suggested that Mycoplasma ovipneumoniae FJ01-CL was the pathogen that caused the caprine mycoplasmal pneumonia in the province for the first time.关键词
绵羊肺炎支原体/分离/鉴定/福建Key words
Mycoplasma ovipneumoniae/isolation/identification/Fujian分类
农业科技引用本文复制引用
江锦秀,林甦,林裕胜,江斌,胡奇林..绵羊肺炎支原体 FJ01-CL 株的分离和鉴定[J].福建农业学报,2015,(5):430-434,5.基金项目
福建省科技计划项目---省属公益类科研院所基本科研专项（2014R1023-6）；福建省科技创新平台建设项目 （）
