福建农业学报Issue(5):522-527,6.
基因组编辑技术及其在植物遗传改良上的应用
Genome Editing and Its Application for Genetic Improvements of Plants
摘要
Abstract
Technology of precise genome editing based on the transcription activator-like effector nucleases (TALENs)and the type II clustered regularly interpaced short palindromic repeats (CRISPR/Cas9 )has been a powerful auxiliary tool for research on genetic functions and improvements of plants.TALENs and CRISPR/Cas9 can induce a double strand break (DSB)between the bound sequence resulting in cellular repairs on DSB through either the homologous recombination (HR)or non-homologous end joining (NHEJ )pathway prior to making modifications on the target genome.This article reviews the information relevant to TALENs and CRISPR/Cas9 based on the structure characteristics,action principle and applications of target modification on plants.Potential applications of the advanced technology were also discussed.关键词
植物/基因组编辑技术/TALENs/CRISPR/Cas9Key words
plants/genome editing/TALENs/CRISPR/Cas9分类
生物科学引用本文复制引用
林雅容,周淑芬,朱义旺,孙庆山,陈惠妹,王锋..基因组编辑技术及其在植物遗传改良上的应用[J].福建农业学报,2015,(5):522-527,6.基金项目
福建省自然科学基金项目 ()