福建水产Issue(6)：419-427,9.DOI:10.14012/j.cnki.fjsc.2014.06.001
一株斜带石斑鱼（Epinephelus coioides）病原菌的分离与鉴定
Isolation and identification of a bacterial pathogen from Epinephelus coioides
摘要
Abstract
Serious disease outbreak occurred in a groupers Epinephelus coioides culture farm in Jinjiang, Fu- <br> jian province in July 2011. The weight of diseased groupers was 3 ~10 g. The characteristics of diseased grouper were observed, displaying body colour darkening, feeding refused, swimming slowly, and redness of the liver and spleen spot. Two strains of bacteria were isolated from the liver and spleen of moribund groupers and designated as strain 201107-I and 201107-II in July 2011 . All the two isolates were identified as Vibrio harveyi by physiological and biochemical tests combining with 16S rRNA gene sequence analysis. Artificial in-jection demonstrated that strain 201107-I and 201107-II were both pathogenic to E. coioides. Strain 201107-II showed the stronger toxicity to E. coioides and was used to the subsequent analysis. Three experimental types of artificial infections were conducted, muscle injection, celiac injection and wound immersion, to con-firm the toxicity of strain 201107-II , and the half lethal dose ( LD50 ) to E. coioides was 4. 3 × 104 , 2. 9 × 105 and 5. 4 × 106 cfu/g fish weight, respectively. We also isolated a bacterial strain from this artificial infected E. coioides, and this strain was identified as the same strain as the 201107-II using traditional physiological and biochemical characterization along with molecular 16S rRNA gene analysis. The sensitivity tests of anti-bacterial agents indicated that the bacterial strain 201107 -II was sensitive to erythromycin, spectinomycin, chloramphenicol, and other 13 agents; middling sensitive to furazolidone, clarithromycin, and ciprofloxacin and other 6 agents;resistant to oxacillin, penicillin G, vancomycin and other 10 agents. This study will pro-vide necessary data about the sustainable and healthy development of E. coioides culture.关键词
斜带石斑鱼/哈维弧菌/细菌鉴定/药敏Key words
Epinephelus coioides/Vibrio harveyi/bacterial identification/sensitivity test to antibacterial a-gents分类
农业科技引用本文复制引用
林克冰,吴建绍,黄兆斌,葛辉,朱志煌,孙敏秋..一株斜带石斑鱼（Epinephelus coioides）病原菌的分离与鉴定[J].福建水产,2014,(6):419-427,9.基金项目
福建省海洋与渔业厅项目“斜带石斑鱼苗种期病害防治技术研究”[闽海渔科(2007)004] （2007）
