福建畜牧兽医Issue(2)：18-19,20,3.
液相色谱串联质谱法在检测鸡肉中环丙沙星残留的应用
Determination of the residues of ciprofloxacin in chicken by high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS /MS) method was developed for the quantitative determination of Ciprofloxacin respectively in the chicken. The ciprofloxacin in chicken were deproteinated with EDTA-Na Mcllvaine buffer followed by liquid-liquid extraction. Further clean-up was performed by solid phase extraction (SPE) through mixed mode columns (PEP-2 SPE). The separation of the compounds was carried on a Agilent Eclipse Plus C18 column (C18, 3.5μm× 100 mm×2.1 mm(id) by a gradient elution using 0.1%formic acid acetonitrile and 0.1%formic acid water as mobile phases. The analyzes were detected by tandem mass spectrometry in multiple reaction monitoring (MRM) mode with negative electrospray ioniza﹣tion. The ciprofloxacin quantified by internal standard method. The linear ranges were 1.0~20.0 μg/L and the correlation coefficients were not less than 0.992. The average recoveries and relative standard deviations ranged from90%~110%and 3%~10%respectively at the spiked levels of 1,2 and 10 μg/kg for the both markers. The limit of quantitation (LOQ) was 1 μg/kg. The method is sensitive,ac﹣curate and suitable for the determination and confirmation of chloramphenicols in chicken and eggs.关键词
液相色谱串联质谱/环丙沙星/残留Key words
high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS)/ciprofloxacin/residue chicken引用本文复制引用
吕小玲,管祖东,黄丽琴,李文迹,罗忠宝..液相色谱串联质谱法在检测鸡肉中环丙沙星残留的应用[J].福建畜牧兽医,2015,(2):18-19,20,3.基金项目
福建省区域科技重大项目（2012N3011）“规模化白羽肉鸡肉品生产兽药残留监控技术的研究与应用”资助。 （）
