分析测试学报Issue(9)：1050-1055,6.DOI:10.3969/j.issn.1004-4957.2014.09.011
维生素K1的快速高通量光诱导化学发光分析
Rapid and High-throughput Determination of Vitamin K1 by Photo-induced Chemiluminescence Detection
摘要
Abstract
A rapid and high-throughput analytical method was developed for the determination of vita-min K1 using photo-induced chemiluminescence( PICL)detection. This method is based on ultravio-let irradiation(254 nm/15 W)of vitamin K1 to produce a fluorescent product which could be deter-mined by peroxideoxalate chemiluminescence system using chemiluminescence detector. Influence of imidazole on photochemical product structure and chemiluminescence mechanism were studied. Under the optimum conditions,a good linear relationship between chemiluminescence intensity and concentra-tion of VK1 over the range of 0. 007-3. 333 μg·mL-1 was obtained. The detection limit(S/N=3) for vitamin K1 was 2. 2 ng·mL-1,and the relative standard deviation(c=0. 1 μg·mL-1,n=11) was 2. 7% . The method could be used to analyze 96 samples in 10 min,and was successfully ap-plied in the determination of vitamin K1 in injection and human serum by standard addition calibra-tion,with recoveries of 96. 0% -104. 0% . The results showed that the PICL method was rapid, high-throughput,simple,sensitive and selective,and was especially suitable for the analysis of a large number of samples.关键词
维生素K1/高通量/紫外光照/化学发光/人血清Key words
vitamin K1/high-throughput/ultraviolet irradiation/chemiluminescence/human ser-um分类
化学化工引用本文复制引用
李云云,章竹君,张琰图..维生素K1的快速高通量光诱导化学发光分析[J].分析测试学报,2014,(9):1050-1055,6.基金项目
国家自然科学基金资助项目（30872371）；陕西省重点实验室项目 （）
