分析测试学报Issue(9)：1062-1067,6.DOI:10.3969/j.issn.1004-4957.2014.09.013
分散液液微萃取/荧光分光光度法快速测定乳制品中的黄曲霉毒素M1
Determination of Aflatoxin in Dairy Products by Dispersive Liquid-Liquid Microextraction and Fluorescence Spectrophotometry
摘要
Abstract
A simple,rapid,and low-cost method based on dispersive liquid-liquid microextraction (DLLME)and fluorescence enhancement spectrophotometry was developed to detect aflatoxin M1 ( AFM1 )in dairy products. Methylene chloride( CH2 Cl2 )and acetonitrile were used as extracting a-gent and dispersant,respectively. The extract was detected by fluorescence spectrophotometry. Un-der the optimum conditions,good linear relationships were obtained for AFM1 in the concentration range of 0. 05-2. 00μg/L with correlation coefficients( r)of 0. 995 6( milk)and 0. 998 9( milk pow-der). The limits of detection( LOD)were 0. 023 μg/L for milk and 0. 039 μg/L for milk powder. The spiked recoveries were in the range of 76. 7% -95. 7% with relative standard deviations( n=6 ) lower than 5% . The method could be applied in fast detection of aflatoxin M1 in dairy products,and there is no significant difference between this method and the national standard method.关键词
分散液液微萃取/荧光分光光度法/乳制品/黄曲霉毒素M1Key words
dispersive liquid-liquid microextraction( DLLME )/fluorospectrophotometry/dairy products/aflatoxin M1分类
化学化工引用本文复制引用
涂春蓉,董晓娟,马良,张宇昊..分散液液微萃取/荧光分光光度法快速测定乳制品中的黄曲霉毒素M1[J].分析测试学报,2014,(9):1062-1067,6.基金项目
国家自然科学基金项目（31301476）；国家级大学生创新创业训练计划 （）
