分析测试学报Issue(9)：1083-1088,6.DOI:10.3969/j.issn.1004-4957.2014.09.017
高效液相色谱法同时测定化妆品中14种禁用着色剂
Simultaneous Determination of 14 Forbidden Colorants in Cosmetics by High Performance Liquid Chromatography
摘要
Abstract
A high performance liquid chromatographic( HPLC)methord was developed for the simul-taneous determination of 14 forbidden colorants( Metanil Yellow,Solvent Blue 35,Solvent Red 49, Acid Violet 49,Tetramethylpararosaniline Chloride( TMPC),Pigment Orange 5,Pigment Red 53, Pentamethyltriphenylmethane( PMTPE ), Sudan Red Ⅱ, Hexamethylenepararosaniline Chloride (HMPC),Solvent Red 24,Rhodamine B,Disperse Yellow 3,Sudan Red Ⅰ)in cosmetics. Cos-metic samples were extracted and dispersed with tetrahydrofuran,and some matrixes were precipitated out after additon of ammonium acetate solution and methanol. After centrifuged and filtered,the ex-tract was separated on an Agilent poroshell 120 EC-C18 column(2. 7 μm,3. 0 mmí100 mm)by a gradient elution with 10 mmol/L ammonium acetate-acetonitrile as mobile phase. Detection wave-lengths were set at 416,514,590 nm,respectively. The qualitative analysis was based on chroma-tographic retention time and UV spectrum,and the quantitative analysis was performed by the stand-ard curve method of peak area. The linear ranges of 14 analytes were in the range of 0. 2-20 μg/mL,with correlation coefficients more than 0. 999. The limits of quantitation( LOQs)were in the range of 3-10 μg/g. The average recoveries at three spiked levels ranged from 91. 3% to 110. 9%with RSDs less than 10% . This method was accurate,simple,sensitive and reliable,and could be used for the analysis of 14 forbidden colorants in cosmetics.关键词
禁用着色剂/化妆品/高效液相色谱Key words
forbidden colorants/cosmetics/high performance liquid chromatography(HPLC)分类
化学化工引用本文复制引用
毛希琴,任国杰,李春玲..高效液相色谱法同时测定化妆品中14种禁用着色剂[J].分析测试学报,2014,(9):1083-1088,6.基金项目
国家质检总局公益性行业科研专项项目 （）
