分析测试学报Issue(10)：1184-1188,5.DOI:10.3969/j.issn.1004-4957.2014.10.015
高效液相色谱-串联质谱法测定辣椒及辣椒制品中金刚烷胺残留
Determination of Amantadine Residues in Pepper and Pepper Products by High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry
摘要
Abstract
A high performance liquid chromatography tandem mass spectrometric( HPLC-MS/MS ) method was developed for the determination of amantadine residues in pepper and pepper products. After extracted with 1% trichloroacetic acid solution-methanol( 1 : 1 )and purified by an Oasis-MCX SPE cartridge,the samples were sepatated on an Atlantis T3 C18 column(3. 0 μm,2. 1 mm × 150 mm)by gradient elution. The electrospray was operated in positive mode and the samples were monitored under the multiple reaction monitoring( MRM)mode. The calibration curves showed good linearity in the range of 1. 0-50μg/kg. The quantitation limit was 1. 0μg/kg for fresh pepper,and 5. 0 μg/kg for dried pepper and chili products. The recoveries of amantadine in fresh pepper at spiked levels of 1. 0,2. 0,5. 0 μg/kg were 72. 2% -98. 2% ,with relative standard deviations ( RSDs,n=6)of 1. 2% -4. 6% ,and recoveries in dry pepper and chili products at spiked levels at 5. 0,10. 0,25. 0 μg/kg were 64. 2% -81. 6% ,with RSDs(n=6)of 1. 3% -6. 3% . With ad-vantages of simple operation,high sensitivity,good reproducibility,the method could be used for the detection of amantadine residues in the pepper and pepper products.关键词
高效液相色谱-串联质谱/金刚烷胺/辣椒/辣椒制品Key words
high performance liquid chromatography - tandem mass spectrometry( HPLC - MS/MS)/amantadine/pepper/pepper products分类
化学化工引用本文复制引用
胡巧茹,周长朋,张玉春,崔嘉,鞠玲燕,杨丽君..高效液相色谱-串联质谱法测定辣椒及辣椒制品中金刚烷胺残留[J].分析测试学报,2014,(10):1184-1188,5.基金项目
国家科技支撑计划项目（2012BAK08B01）；质检公益性行业科研专项资助（201310143）；国家质检总局科技计划项目 （）
