分析化学Issue(6)：853-858,6.DOI:10.3724/SP.J.1096.2014.30884
基于石墨烯和蒽醌-2-磺酸钠的 Pb2+核酸适体电化学传感器
Graphene and Anthraquinone-2-sulfonic Acid Sodium Based Electrochemical Aptasensor for Pb2+
摘要
Abstract
An electrochemical aptasensor for Pb2+ was constructed based on the layer-by-layer assembly of graphene (GR) and anthraquinone -2-sulfonic acid sodium salt (AQMS) on the surface of Pb2+ aptamer. The mercapto-modified Pb2+ aptamer was first anchored on a gold electrode. Then the highly conductive material of GR was adsorbed on apt through the unique π-π stacking interaction, which was further used for the assembly and signal amplification of the electroactive AQMS. Upon interaction with Pb2+ ions, the apt on the aptasensor undergone conformational switch from a single-stranded form to the G-quadruplex structure, causing the GR assembled with AQMS releasing from the electrode surface into solution. As a result, the electrochemical signal of AQMS on the aptasensor was substantially reduced. Base on this concept, a useful platform for detection of Pb2+ ions was constructed. Under the optimal experimental conditions, the attenuation of peak currents (△Ipa ) showed linear relationship with the logarithm of Pb2+ concentrations (lgCPb2+) over the range from 5. 0×10-10 to 5. 0×10-8 mol/ L. The detection limit was estimated to be 6. 0×10-11 mol/ L.关键词
铅离子/适体传感器/石墨烯/蒽醌-2-磺酸钠盐Key words
Lead ion/Aptasensor/Graphene/Anthraquinone-2-sulfonic acid sodium salt引用本文复制引用
高彩,杭乐,廖晓磊,高飞,汪庆祥..基于石墨烯和蒽醌-2-磺酸钠的 Pb2+核酸适体电化学传感器[J].分析化学,2014,(6):853-858,6.基金项目
本文系国家自然科学基金(No.21275127)、福建省自然科学基金( No.2011J01059)、“福建省高等学校新世纪优秀人才支持计划”(No. JA12204)项目资助 （No.21275127）
