分析化学Issue(7)：991-996,6.DOI:10.11895/j.issn.0253-3820.131160
黄嘌呤和尿酸在修饰电极上的电化学行为及测定研究
Study on Electrochemical Behaviors and Determination of Xanthine and Uric Acid on Modified Glassy Carbon Electrode
摘要
Abstract
The silver doped poly ( L-lysine ) modified glassy carbon electrode was fabricated by cyclic voltammetry, the surface of the electrode was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. The electrochemical behaviors and the simultaneous detection of xanthine and uric acid were studied by cyclic voltammetry and differential pulse voltammetry. The results indicated that this modified electrode exhibited excellent electrocatalytic activity towards the oxidation of xanthine and uric acid. The stable oxidation peaks of xanthine and uric acid appeared with the peak potential of 0. 980V and 0. 600V respectively at the modified electrode in pH 3. 0 phosphate buffer solution. The oxidation peaks of xanthine and uric acid were separated at 380 mV. Under the optimum conditions, the linear ranges for the determination of xanthine and uric acid were 1 . 00 × 10-6-2 . 50 × 10-4 mol/L respectively by differential pulse voltammetry. The detection limits were 5. 0×10-7mol/L. The method has been applied to the simultaneous detection of xanthine and uric acid in healthy human urine with satisfactory results.关键词
银/L-赖氨酸/修饰电极/黄嘌呤/尿酸/循环伏安法/差分脉冲伏安法Key words
Silver/L-lysine/Modified electrode/Xanthine/Uric acid/Cyclic voltammetry/Differential pulse voltammetry引用本文复制引用
孙凯,孙登明..黄嘌呤和尿酸在修饰电极上的电化学行为及测定研究[J].分析化学,2014,(7):991-996,6.基金项目
本文系安徽省高校省级自然科学研究重点项目基金(No. KJ2011A255)资助@@@@This work was supported by the Natural Science Foundation of Anhui Province Ministry of Education (No. KJ2011A255)@@@@This work was supported by the Natural Science Foundation of Anhui Province Ministry of Education (No. KJ2011A255) （No. KJ2011A255）
