分析化学Issue(8)：1088-1093,6.DOI:10.11895/j.issn.0253-3820.131230
细胞代谢组学用于木犀草素抑制MCF-7细胞的机制研究
Metabonomics Study on Luteolin Intervention of Breast Cancer MCF-7 Cells
摘要
Abstract
The metabolic profiles of control and MCF-7 cells treated with luteolin were analyzed separately using gas chromatography/mass spectrometry ( GC/MS ) to study the mechanism of the luteolin treatment on MCF-7 cells. Cell viability assays showed that luteolin had inhibition effect on MCF-7 cells. Partial least square discriminant analysis ( OPLS-DA) was used to process the metabolic data. Since cells in phase of S were increased significantly, we speculated that luteolin had a blocking effect on pentose phosphate pathway of MCF-7 cells, which contributed to its inhibition effect on proliferation of MCF-7 cells.关键词
气相色谱质谱联用/细胞代谢组学/MCF-7 细胞/细胞周期/乳腺癌/木犀草素Key words
Gas chromatography/mass spectrometry/Cell metabonomics/MCF-7 cells/Cell cycle/Breast cancer/Luteolin引用本文复制引用
史栋栋,王桂明,况媛媛,彭章晓,王彦,谷雪,阎超..细胞代谢组学用于木犀草素抑制MCF-7细胞的机制研究[J].分析化学,2014,(8):1088-1093,6.基金项目
国家自然科学基金项目(Nos.21175092,21105064),国家重大科学仪器设备开发专项(Nos.2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210),上海市自然科学基金项目(No.12ZR1413600)基金资助项目@@@@This work was supported by the National Natural Science Foundation of China (Nos.21175092,21105064), the national Major Scientific Instruments and Equipments Special Project (Nos.2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210), and the Nation-al Natural Science Foundation of Shanghai, China (No.12ZR1413600) （Nos.21175092,21105064）
