分析化学Issue(8)：1144-1149,6.DOI:10.11895/j.issn.0253-3820.140191
微悬臂梁适配子传感器检测维埃克斯、沙林及动力学分析
Aptamer-Based Microcantilever Sensor for O-ethyl S-[2-(diisopropylamino) ethyl] methylphosphonothiolate, Sarin Detection and Kinetic Analysis
摘要
Abstract
A new method for O-ethyl S-[2-( diisopropylamino) ethyl] methylphosphonothiolate ( VX) , sarin detection and its kinetic analysis based on piezoresistive microcantilever aptasensor was developed, where VX, sarin aptamers were immobilized on the microcantilever surface by biotin-avidin binding system. A linear relationship between the response voltage and the concentration of VX in the range of 2-60μg/L was obtained. The linear regression equation was △Ue=0. 886C-1. 039 (n=5, R=0. 984, p<0. 001) and the detection limit was 2μg/L ( S/N≥3 ) . A linear relationship between the response voltage and the concentration of sarin in the range of 10-60 μg/L was obtained, the linear regression equation was △Ue=0. 716C-2. 304 ( n=5, R=0 . 996 , p<0 . 001 ) and the detection limit was 10 μg/L ( S/N≥3 ) . The sensor showed no response for O-butyl methylphosphonochloridate, a structural analog of VX and sarin, which indicated high specificity and good anti-interference ability. On this basis, a reaction kinetic model based on receptor-ligand binding and the relationship with output voltage change was established. Response voltage (△Ue ) and response time( t0 ) were obtained from the fitting equation on different concentrations of VX, sarin fitted well with the measured values.关键词
微悬臂梁/适配子传感器/维埃克斯/沙林/动力学分析Key words
Microcantilever/Aptasensor/O-Ethyl S-[2-( diisopropylamino) ethyl] methylphosphonothiolate/Sarin/Kinetic analysis引用本文复制引用
刘志伟,童朝阳,郝兰群,刘冰,穆晞惠,张金平,高川..微悬臂梁适配子传感器检测维埃克斯、沙林及动力学分析[J].分析化学,2014,(8):1144-1149,6.基金项目
本文系总装预研项目资助(No.51326050301)@@@@This work was Supported by the General Armament Department Advance Research Project ( No.51326050301) （No.51326050301）
