分析化学Issue(8)：1150-1155,6.DOI:10.11895/j.issn.0253-3820.140208
直接竞争酶联免疫法测定食品中的丙烯酰胺含量
Direct Competitive Enzyme-Linked Immunosorbent Assay for Detection of Acrylamide in Food Samples
摘要
Abstract
Due to the low molecular weight and simple structure, the production of specific antibodies against acrylamide is unavailable. In this study, a novel hapten was synthesized through the derivatization of acrylamide and 4-mercaptophenylacetic acid. The hapten was then coupled to carrier protein and used to immunize New Zealand rabbits. Polyclonal antibody which showed specific binding to the acrylamide derivative ( hapten) was obtained. The antibody was labeled with horseradish peroxidase ( HRP) and used to develop a direct competitive enzyme-linked immunosorbent assay ( dc-ELISA) . The dc-ELISA was used to determine the content of acrylamide derivative, and then transferred to the content of acrylamide. The assay showed an IC50 value of 45. 49 μg/L, a limit of detection of 3. 0 μg/L and the linear range of 9. 2-195 μg/L for acrylamide. The recovery of acrylamide from spiked food sample was determined ranging from 83 . 6% to 112 . 7%. Good correlations between the results of dc-ELISA and standard HPLC-MS/MS were obtained. The proposed dc-ELISA is suitable for the determination of acrylamide in food samples.关键词
丙烯酰胺/多克隆抗体/直接竞争酶联免疫分析/衍生Key words
Acrylamide/Polyclonal antibody/Direct competitive enzyme-linked immunosorbent assay/Derivatization引用本文复制引用
吴璟,罗林,肖治理,杨金易,孙远明,雷红涛,沈玉栋,王弘,徐振林..直接竞争酶联免疫法测定食品中的丙烯酰胺含量[J].分析化学,2014,(8):1150-1155,6.基金项目
本文系973课题(2012CB720803)和广东省高等学校学科与专业建设专项资金(2013KJCX0027)项目资助@@@@This work was supported by the State Key Development Program for Basic Research of China (No.2012CB720803) （2012CB720803）
