分析化学Issue(9)：1295-1300,6.DOI:10.11895/j.issn.0253-3820.140310
适配体-荧光共振能量转移法检测雌二醇
Aptamer-based Fluorescence Resonance Energy Transfer Method for Determination of Estradiol
摘要
Abstract
A highly sensitive fluorescence spectroscopic method was established for the selective determination of estradiol, which took advantages of the excellent molecular recognition capability of aptamer and the energy transfer between the specific fluorescent groups. The effects of the pH value, buffer constituent and concentration, the concentration of DNA, the experimental temperature and response time on the detection of estradiol were studied. Under the optimal conditions (50 mmol/L BR buffer solution with pH value at 7. 4, 1. 0×10-7 mol/L for each DNA strand, incubation at 45 ℃, response time 19 min), the change of the fluorescence intensity (ΔI) versus the logarithm of the concentration of estradiol ( lgC) was linear over a concentration range from 1. 0×10-11 mol/L to 5. 0×10-9 mol/L with good linear correlation (r=0. 9953). The limit of detection (LOD) was found to be 6. 0×10-12 mol/L (S/N=3). This method was successfully applied to the detection of estradiol in human urine, with the recovery in the range of 94. 0%-103. 5%. This method showed good precision and accuracy.关键词
适配体/荧光共振能量转移/雌二醇Key words
Aptamer/Fluorescence resonance energy transfer/Estradiol引用本文复制引用
蒋晓华,丁文捷,栾崇林..适配体-荧光共振能量转移法检测雌二醇[J].分析化学,2014,(9):1295-1300,6.基金项目
广东省高等学校“千百十工程”人才培养专项基金( No.01080106),深圳市战略性新兴产业发展专项资金项目(No.2113K3070038)资助 （ No.01080106）
