分析化学Issue(9)：1314-1319,6.DOI:10.11895/j.issn.0253-3820.140390
液相色谱-氢化物发生原子荧光光谱法测定富硒酵母中硒的形态
Quantitative Determination of Selenium Species in Se-enriched Yeast Using Liquid Chromatography-Hydride Generation Atomic Fluorescence Spectrometry
肖志明 1宋荣 1贾铮 1李阳 1樊霞1
作者信息
- 1. 中国农业科学院农业质量标准与检测技术研究所，国家饲料质量监督检验中心(北京)，北京 100081
- 折叠
摘要
Abstract
A rapid and sensitive method has been developed for the simultaneous determination of four selenium species Se(Ⅵ), Se(Ⅵ), selenomethionine, and Se-methylselenocysteine in Se-enriched yeast by liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS). The isolation of the analytes from yeast samples was accomplished by proteaseⅩⅣ and trypsin enzymatic digestion. The target compounds were separated on a PRP-X100 anion exchange column and analyzed by HG-AFS. The mobile phase was 20 mmol/L (NH4)2HPO4. Good linearity was obtained for all the selenium species, with linear correlation coefficients higher than 0. 9996. The LODs of the four species were between 0. 5 and 5. 0 μg/kg. Average recoveries for the four analytes were in the ranges of 82 . 5%-101 . 2%, with intra-and inter-day RSD lower than 8. 6% and 14. 5, respectively. The proposed analytical method is simple, sensitive, with low operation cost, making it applicable for the determination of the selenium species in Se-enriched feeds.关键词
液相色谱/氢化物发生原子荧光光谱/富硒酵母/硒形态Key words
Liquid chromatography/Hydride generation atomic fluorescence spectrometry/Se-enriched yeast/Selenium species引用本文复制引用
肖志明,宋荣,贾铮,李阳,樊霞..液相色谱-氢化物发生原子荧光光谱法测定富硒酵母中硒的形态[J].分析化学,2014,(9):1314-1319,6.
