分析化学Issue(9)：1387-1388,2.DOI:10.11895/j.issn.0253-3820.140129
分散式固相萃取结合高效液相色谱法快速测定油脂制品中苯并（a）芘
Determination of Benzo(a) pyrene Residues in Edible Oil and Oil Products by High Performance Liquid Chromatography with Dispersive Solid Phase Extraction
杨悠悠 1王浩 2刘佟 1杨永坛2
作者信息
- 1. 中粮营养健康研究院
- 2. 食品安全与营养健康北京市重点实验室，北京100020
- 折叠
摘要
Abstract
A dispersive solid phase extraction method combined with reversed phase liquid chromatography was investigated for the determination of benzo ( a ) pyrene ( BaP ) residues in edible oil and oil products. The developed “one-step” sample preparation method integrated with the adsorbent activation, sample extraction and sample cleaning. Using Alumia-N as the adsorbent, good cleaning effect and high recovery ranging from 81. 5% -97. 5% was achieved. In addition, coupled with fluorescence detector (FLD), high sensitivity was obtained with LOD of 1. 0 μg/kg (S/N=3). Furthermore, SPSS was used to design orthogonal optimization experiments and establish the optimal condition. Under the optimized condition, the standard calibration curve was linear over the concentration range of 0. 5-10. 0μg/L, with the regression efficiency (R2) of 0. 9997. The relative standard deviation (RSD) of peak area was between 2. 6%-4. 9%, showing good repeatability and the reliability.关键词
Benzo( a) pyrene/High performance liquid chromatography/Dispersive solid phase extraction/Oil productKey words
Benzo( a) pyrene/High performance liquid chromatography/Dispersive solid phase extraction/Oil product引用本文复制引用
杨悠悠,王浩,刘佟,杨永坛..分散式固相萃取结合高效液相色谱法快速测定油脂制品中苯并（a）芘[J].分析化学,2014,(9):1387-1388,2.
