分析化学Issue(11)：1568-1573,6.DOI:10.11895/j.issn.0253-3820.140279
用于细胞排列的介电泳微流控芯片制备与实验研究
Preparation and Experimental Study on Dielectrophoresis- Based Microfluidic Chip for Cell Patterning
摘要
Abstract
Adielectrophoresis-basedmicrofluidicchipappliedtocellspatterningisdesignedandfabricated, and it demonstrates non-contact and batch manipulation of cells. The microfluidic chip employs a PDMS microchannel and two ITO electrodes, which are designed as a"step" shape. The distribution of electric field caused by the microelectrodes is simulated by finite element simulation software, COMSOL. The position of the maximum intensity of electric field is also determined. The ITO microelectrodes and the PDMS microchannel are fabricated using MEMS fabrication process. After oxygen plasma surface treatment, the PDMS microchannel and glass substrate with the ITO microelectrodes are aligned and bonded to form experimental microfluidic chip. Through DEP experiment with the varying frequencies, DEP response of yeast cells is examined, and the electric field frequency of the both positive and negative DEP responses are confirmed. The results showed that yeast cells in solution conductivity of 60 μS/cm had negative DEP movement at the frequency of 1 kHz to 10 kHz, positive DEP movement at the 500 kHz to 10 MHz, and no DEP movement at the 50 kHz. Under the condition of the sinusoidal potential of 8Vp-p and the electric field frequency of 5 MHz, the yeast cells were aligned into chains along the "step" edge of microelectrodes.关键词
介电泳/微流控芯片/有限元仿真/细胞排列Key words
Dielectrophoresis/Microfluidic chip/Finite element simulation/Cell patterning引用本文复制引用
张洋,张晓飞,白国花,方明,谭秋林,熊继军,孙东..用于细胞排列的介电泳微流控芯片制备与实验研究[J].分析化学,2014,(11):1568-1573,6.基金项目
This work was supported by the National Natural Science Foundation of China (Nos.51205373,51275491) （Nos.51205373,51275491）
